University of Wisconsin–Madison

Urchins

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Adult L. pictus & S. purpuratus
Adult L. pictus & S. purpuratus [J. Hardin]
L. variegatus unfertilized egg

L. variegatus unfertilized egg [J. Hardin]
L. variegatus fertilized egg
L. variegatus fertilized egg [J. Hardin]
L. variegatus 2-cell
L. variegatus 2-cell
[J. Hardin]
L. variegatus 4-cell
L. variegatus 4-cell [J. Hardin]
L. variegatus 8-cell
L. variegatus 8-cell [J. Hardin]
L. variegatus 16-cell
L. variegatus 16-cell [J. Hardin]
L. variegatus 16-cell, vegetal view
L. variegatus 16-cell, vegetal view [J. Hardin]
Isolated L. variegatus 16-cell blastomeres
Isolated L. variegatus 16-cell blastomeres [J. Hardin]
L. variegatus late 16-cell
L. variegatus late 16-cell [J. Hardin]
L. variegatus 32-cell
L. variegatus late 32-cell [J. Hardin]
L. variegatus 32-cell (vegetal)
L. variegatus 32-cell (vegetal) [J. Hardin]
L. variegatus hatched blastula
L. variegatus hatched blastula [J. Hardin]
L. variegatus mesenchyme blastula
L. variegatus mesenchyme blastula [J. Hardin]
L. variegatus late gastrula
L. variegatus late gastrula [J. Hardin]
L. variegatus late gastrula - oral view
L. variegatus late gastrula – oral view [J. Hardin]
L. variegatus prism stage
L. variegatus prism stage [J. Hardin]
L. variegatus late prism stage
L. variegatus late prism stage [J. Hardin]
L. variegatus pluteus
L. variegatus pluteus [J. Hardin]
S. purpuratus early plutei - polarization microscopy
S. purpuratus early plutei – polarization microscopy [J. Hardin}
S. purpuratus early plutei - polarization microscopy
S. purpuratus early plutei – polarization microscopy [J. Hardin}
L. variegatus primary mesenchyme
L. variegatus primary mesenchyme [J. Hardin]
L. pictus early gastrula - phalloidin
L. pictus early gastrula – phalloidin [J. Hardin]
L. pictus vegetal plate - SEM
L. pictus vegetal plate – SEM [J. Hardin]
L. pictus vegetal plate - FM4-64
L. pictus vegetal plate – FM4-64 [J. Hardin]
L. variegatus - Mab183 treated
L. variegatus – Mab183 treated [J. Hardin]
L. pictus gastrulae - labeled clones in vegetal plate
L. pictus gastrulae – labeled clones in vegetal plate [J. Hardin]
L. pictus gastrulae
L. pictus gastrulae [J. Hardin]
L. variegatus late gastrula (ventral)
L. variegatus late gastrula - LvS1 in situ
L. variegatus late gastrula – LvS1 in situ
L. variegatus mid-gastrula in Nitex mesh [J. Hardin]
L. variegatus mid-gastrula in Nitex mesh [J. Hardin]
L. variegatus gastrula in Nitex mesh
L. variegatus late gastrula in Nitex mesh [J. Hardin]
L. variegatus late gastrula in capillary tube
L. variegatus late gastrula in capillary tube [J. Hardin]
L. pictus exogastrula
L. pictus exogastrula [J. Hardin]
L. variegatus prism - Endo1
L. variegatus prism – Endo1 [J. Hardin]
L. variegatus pluteus - oral view
L. variegatus pluteus – oral view [J. Hardin]
L. variegatus pluteus - aboral view
L. variegatus pluteus – aboral view [J. Hardin]
L. variegatus pluteus - polarization microscopy
L. variegatus pluteus – polarization microscopy [J. Hardin]
L. variegatus late gastrula - vegetal view

L. variegatus late gastrula – vegetal view [J. Hardin]
L. variegatus - snail (red) & PMCs (green)
L. variegatus – snail (red) & PMCs (green) [J. Hardin]
L. variegatus gastrula - 6a3 staining
L. variegatus gastrula – 6a3 staining [J. Hardin]
L. variegatus late gastrula - 1g8 staining + ECM
L. variegatus late gastrula – 1g8 staining + ECM [J. Hardin]
L. variegatus pluteus - NiCl2 - polarization microscopy
L. variegatus pluteus – NiCl2 – polarization microscopy [J. Hardin]
L. variegatus pluteus with NiCl2 treated PMCs
L. variegatus pluteus with NiCl2 treated PMCs [J. Hardin]
L. variegatus NiCl2 treated pluteus with normal PMCs
L. variegatus NiCl2 treated pluteus with normal PMCs [J. Hardin]
L. variegatus gastrula - NiCl2 treated (vegetal) [J. Hardin]
L. variegatus gastrula – NiCl2 treated (vegetal) [J. Hardin]
L. variegatus prism - NiCl2 treated (polarization)
L. variegatus prism – NiCl2 treated (polarization) [J. Hardin]
L. variegatus prism - NiCl2 treated (1g8 stained)
L. variegatus prism – NiCl2 treated (1g8 stained) [J. Hardin]
L. variegatus veg2 clone induces skeleton
L. variegatus veg2 clone induces skeleton [J. Hardin]
L. variegatus veg2 clone induces skeleton
L. variegatus veg2 clone induces skeleton [J. Hardin]
L. variegatus pluteus - animal pole removed at gastrula
L. variegatus pluteus – animal pole removed at gastrula [J. Hardin]
L. variegatus animal pole + PMCs
L. variegatus animal pole + PMCs [J. Hardin]